Journal article
Altered microRNA profiles during early colon adenoma progression in a porcine model of familial adenomatous polyposis.
- Stachowiak M Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 60-637 Poznan, Poland.
- Flisikowska T Chair of Livestock Biotechnology, Technische Universität München, 85354 Freising, Germany.
- Bauersachs S Institute of Agricultural Sciences, Animal Physiology, ETH Zurich, CH-8092 Zurich, Switzerland.
- Perleberg C Chair of Livestock Biotechnology, Technische Universität München, 85354 Freising, Germany.
- Pausch H Institute of Agricultural Sciences, Animal Genomics, ETH Zurich, CH-8092 Zurich, Switzerland.
- Switonski M Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 60-637 Poznan, Poland.
- Kind A Chair of Livestock Biotechnology, Technische Universität München, 85354 Freising, Germany.
- Saur D Klinikum Rechts der Isar II, Technische Universität München, 81675 Munich, Germany.
- Schnieke A Chair of Livestock Biotechnology, Technische Universität München, 85354 Freising, Germany.
- Flisikowski K Chair of Livestock Biotechnology, Technische Universität München, 85354 Freising, Germany.
- 2017-12-10
Published in:
- Oncotarget. - 2017
English
MicroRNAs are dysregulated in various cancers including colorectal cancer, and are potential useful biomarkers of disease development. We used next generation sequencing to investigate miRNA expression profiles in low- and high-grade intraepithelial dysplastic polyps from pigs carrying a mutation in the adenomatous polyposis coli tumour suppressor (APC1311 , orthologous to human APC1309 ) that model an inherited predisposition to colorectal cancer, familial adenomatous polyposis. We identified several miRNAs and their isomiRs significantly (P < 0.05) differentially expressed between low and high-grade intraepithelial dysplastic polyps. Of these, ssc-let-7e, ssc-miR-98, ssc-miR-146a-5p, ssc-miR-146b, ssc-miR-183 and ssc-miR-196a were expressed at higher level and ssc-miR-126-3p at lower level in high-grade intraepithelial dysplastic polyps. Functional miRNA target analysis revealed significant (P < 0.001) over-representation of cancer-related pathways, including 'microRNAs in cancer', 'proteoglycans in cancer', 'pathways in cancer' and 'colorectal cancer'. This is the first study to reveal miRNAs associated with premalignant transformation of colon polyps.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.18632/oncotarget.21774
- PMID 29221194
- Persistent URL
- https://roar.hep-bejune.ch/global/documents/167741
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