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Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant.

  • Folcher M Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Oesterle S Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Zwicky K Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Thekkottil T Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Heymoz J Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Hohmann M Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Christen M Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Daoud El-Baba M Département Génie Biologique, Institut Universitaire de Technologie (IUTA), 74 Boulevard Niels Bohr, F-69622 Villeurbanne, France.
  • Buchmann P Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
  • Fussenegger M 1] Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland [2] Faculty of Science, University of Basel, Mattenstrasse 26, CH-4058 Basel, Switzerland.
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  • 2014-11-12
Published in:
  • Nature communications. - 2014
Alkaline Phosphatase
Animals
Brain-Computer Interfaces
Cyclic GMP
Electroencephalography
Female
Gene Expression
Humans
Implants, Experimental
Mice
Optogenetics
Signal Transduction
Transcription, Genetic
Transgenes
Wireless Technology
English Synthetic devices for traceless remote control of gene expression may provide new treatment opportunities in future gene- and cell-based therapies. Here we report the design of a synthetic mind-controlled gene switch that enables human brain activities and mental states to wirelessly programme the transgene expression in human cells. An electroencephalography (EEG)-based brain-computer interface (BCI) processing mental state-specific brain waves programs an inductively linked wireless-powered optogenetic implant containing designer cells engineered for near-infrared (NIR) light-adjustable expression of the human glycoprotein SEAP (secreted alkaline phosphatase). The synthetic optogenetic signalling pathway interfacing the BCI with target gene expression consists of an engineered NIR light-activated bacterial diguanylate cyclase (DGCL) producing the orthogonal second messenger cyclic diguanosine monophosphate (c-di-GMP), which triggers the stimulator of interferon genes (STING)-dependent induction of synthetic interferon-β promoters. Humans generating different mental states (biofeedback control, concentration, meditation) can differentially control SEAP production of the designer cells in culture and of subcutaneous wireless-powered optogenetic implants in mice.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://roar.hep-bejune.ch/global/documents/183999
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