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Journal article

Sonic hedgehog guides post-crossing commissural axons both directly and indirectly by regulating Wnt activity.

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  • 2010-08-20
Published in:
  • The Journal of neuroscience : the official journal of the Society for Neuroscience. - 2010
Animals
Avian Proteins
Axons
COS Cells
Cell Movement
Chemotaxis
Chick Embryo
Chlorocebus aethiops
Coculture Techniques
Hedgehog Proteins
Spinal Cord
Wnt Proteins
English After midline crossing, axons of dorsolateral commissural neurons turn rostrally into the longitudinal axis of the spinal cord. In mouse, the graded distribution of Wnt4 attracts post-crossing axons rostrally. In contrast, in the chicken embryo, the graded distribution of Sonic hedgehog (Shh) guides post-crossing axons by a repulsive mechanism mediated by hedgehog-interacting protein. Based on these observations, we tested for a possible cooperation between the two types of morphogens. Indeed, we found that Wnts also act as axon guidance cues in the chicken spinal cord. However, in contrast to the mouse, Wnt transcription did not differ along the anteroposterior axis of the spinal cord. Rather, Wnt function was regulated by a gradient of the Wnt antagonist Sfrp1 (Secreted frizzled-related protein 1) that in turn was shaped by the Shh gradient. Thus, Shh affects post-crossing axon guidance both directly and indirectly by regulating Wnt function.
Language
  • English
Open access status
bronze
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Persistent URL
https://roar.hep-bejune.ch/global/documents/235931
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