Journal article
Enzyme and protein composition of myelin isolated in the presence of EGTA.
- Burgisser P Laboratoire de Neurochimie, Service de Pédiatrie, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, Switzerland.
- Matthieu JM
- 2010-05-22
Published in:
- Neurochemistry international. - 1981
English
The efficiency of ethyleneglycol-bis (?-amino-ethyl ether) N,N?-tetra-acetic acid (EGTA) in removing possible contamination from myelin was tested. Myelin fractions were isolated in the presence or absence of EGTA. An axolemma-enriched fraction was also prepared. Gel electrophoresis showed no important alteration of the protein pattern of myelin treated with EGTA. Only a minor band of about 41,000 daltons was selectively removed when EGTA was used during the two density gradient and differential centrifugation steps. EGTA, when used in the final washes, did not remove this band. It was absent from axolemma-enriched fractions. Different hypotheses are considered to explain these findings.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/0197-0186(81)90049-8
- PMID 20487808
- Persistent URL
- https://roar.hep-bejune.ch/global/documents/259013
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